Generate human immunological insights with a comprehensive solution for vaccine development

Research by M. Scholaert1,2, M. Peries2, E. Braun2, J. Martin1, N. Serhan1, A. Loste1, A. Bruner1, L. Basso1, E. Merle2, A. Trevisiol2, E. Pagès2 and N. Gaudenzio1,2
1Toulouse Insitute for Infectious and Inflammatory Diseases (Infinity), 2Genoskin

High-dimensional profiling of immune response to vaccines using bio-stabilized and injectable natural human skin.

Vaccines are often developed using a combination of congenic mouse experiments, in vitro culture of human peripheral blood cells and, for some of them, data-driven biosimulation platforms. While such experimental settings have been very useful, they often fail to recapitulate the complexity of the human immune system found in the tissue at the site of injection. The skin is composed of structural cells that tightly interact with more than 17 different immune cells to form a unique ecosystem.

We have developed a proprietary method to maintain ex vivo human skin biopsies (Hyposkin®) alive, functional and fully immunocompetent for a prolonged period of time. Built on Hyposkin® technology, we have designed a general framework to study the early steps of human immune responses to vaccines and adjuvants in their natural environment at the site of injection.

Multi-modal immunoprofiling of skin response to vaccines using VaxSkin

Combining 3-D two photon imaging, multiplexed cytokines dosage and single cell RNA sequencing, here we show that both immune and structural compartments in HypoSkin® stay alive and functional for a 10 days period and that the injection of the Moderna vaccine against COVID19 triggers the release of a specific signature of cytokines and chemokines, among which MDC, TARC and MCP-1, which are well known to be involved in T cell and monocyte recruitment. Finally, the longitudinal single cell analysis of skin-resident immune cells transcriptome upon injection showed a time-dependent modulation of myeloid and lymphoid cells activation status, strongly suggesting the generation of a coordinated immune response at the site of injection.

The VaxSkin® platform thus provides a unique framework for the study of the early steps of immune response to drugs in its native environment and, combined with different technologies, it might help to maximize specific immune targeting or evaluate adjuvant potency ahead of clinical trials.

Please fill in the form below to access our poster.

After submitting this form, you will receive an email containing the link to access our poster. We’d like you to know that we take data confidentiality very seriously. The information you enter in this form will only be collected and processed to answer your request and facilitate our mutual communication. Your data will not be sold nor given to any third parties. You have the right to access, rectify or delete your personal data. To find out more about how Genoskin manages your personal data, please consult our privacy policy.

    First Name

    Last Name





    Need more info? Please contact us!